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Animal studies have also reported elevated resting energy expenditure
The assay allows for MDA to be measured from an MDA-TBA adduct forming from the reaction of MDA and TBARS under high temperature (90-100C) and acidic conditions
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Structural characterization further demonstrated that FSEN1 interacts with a critical phenylalanine residue that is absent in mouse FSP1, explaining the human-specific activity of FSEN1
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